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Identifying the uncinate fasciculus (UF) in DTIStudio using a multiple ROI approach
Written 8/8/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email 
if you have any questions. This protocol explains how to identify the uncinate fasciculus (UF) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see http://www.loni.ucla.edu/~narr/protocols.php or the related protocols below.
Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.
To identify the UF:
| First ROI: Identify the most posterior coronal slice in which the green fibers connecting the temporal and frontal lobes separate (marked by the arrow; 1). This slice is typically located at the anterior portion of the fornix in the midsagittal view. Select all fibers in the temporal lobe (2). | |
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 2 |
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Second ROI: In the same coronal slice, select the fibers projecting to the frontal lobe. If these fibers are not clearly delineated, select a slice slightly more anterior when they become visible. |
| * Be sure to remove all fibers that are clearly not a part of the tract. | |
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Sagittal view of the uncinate fasciculus. |
References
- Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Click here to view.