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Identifying the arcuate fasciculus (AF) in DTIStudio using a multiple ROI approach
Written 8/8/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email 
if you have any questions. This protocol explains how to identify the arcuate fasciculus (AF) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see http://www.loni.ucla.edu/~narr/protocols.php or the related protocols below.
Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.
To identify the AF:
| First ROI: Identify the most inferior axial slice in which the fornix is visible. In the color map, the fornix is a blue circular structure (marked by the arrow; 1). Identify the coronal slice in which the indicated green fibers are most intense (2). Select all green fibers in this region (3). | ||
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Second ROI: Using the axial slice identified for the first ROI, select the bundle of fibers projecting laterally to the sagittal stratum (SS). * Be sure to remove all fibers that are clearly not a part of the tract. | |
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Sagittal view of the arcuate fasciculus. |
References
- Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Click here to view.